A FASTQ file is a text file that contains the sequence data from the clusters that pass filter on a flow cell. Illumina sequencing instruments generate per-cycle BCL basecall files as primary ...

snRNA-seq data is saved in bcl format, to convert it into fastq file format, two packages are required: cellranger and bcl2fastq2. The conversion command is cellranger mkfastq. The FASTQ output ...