When biotinylated peptides were used ... At the 8-hour time point, cells were washed twice and stained for CD3 surface antigen and for intracellular IL-2 (PharMingen, San Diego, California).

This expanding list of proteins consists of several fluorescent-labeled target antigens and pre-biotinylated proteins that are especially appropriate for assessing CAR expression. Besides ...

This makes it a perfect tool in protein biochemistry. But producing high-quality biotinylated proteins presents a significant challenge. Scientists usually face technical problems like high batch ...