Baylor College of Medicine4 年
Generation of Knock-in Mice Using Long Single-Stranded DNA (lssDNA) or Double-Stranded DNA ...
For cytoplasmic injections the mix will contain the guide RNA(s), DNA repair template(s), and Cas9 mRNA in nuclease-free buffer at concentrations determined by the core. For pronuclear microinjections ...
Baylor College of Medicine3 个月
CRISPR-assisted ROSA26 Targeting with Double-stranded DNA (dsDNA) in Mouse Zygotes
CRISPR/Cas-initiated HR in mouse embryos will be used to generate founder animals harboring inserted sequence targeted to the ROSA26 locus. The targeting will be done in embryos using microinjection ...