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One class uses different fluorescent dyes incorporated in short oligonucleotide probes specific for the amplified target. The second class consists of dyes that bind double-stranded DNA and become ...

In double-quenched BHQnova Probes the nova quencher is closer to the fluorophore which serves to enchance quenching efficacy. A higher quenching efficiency lowers background fluorescence and ...

Thus, Southern blotting and fluorescence-monitored PCR ... 300 nM reverse primer, 100 nM probe primer and 100–500 ng of template DNA in a 30-μl volume. After initial incubations at 50 °C ...

which labels DNA, RNA and specific proteins with fluorescent probes. This triple-layered technique allowed the researchers to simultaneously capture the location of DNA, RNA and specific proteins ...