Then, to perform PCR, the DNA template that contains the target is added to a tube that contains primers, free nucleotides, and an enzyme called DNA polymerase, and the mixture is placed in a PCR ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

The presence of human waste in sewage overflow or stormwater runoff that ultimately finds its way into rivers and lakes is a ...