However, in fact a 'B6.129' congenic strain is developed with the disrupted 129-derived targeted allele and an unpredictable amount of flanking 129-derived DNA. Often after the targeted allele is ...
Repair reactions involve the excision of chemically altered or mispaired bases from the DNA duplex. Resulting gaps are filled in by DNA polymerases; this reaction leaves a nick at or flanking the site ...