(a) The basic elements of FISH are a DNA probe and a target sequence. (b) Before hybridization, the DNA probe is labeled by various means, such as nick translation, random primed labeling ...

(A) FISH produces a fluorescent signal (red) at the sites of a specific DNA sequence; in this case, a 150-kb segment of chromosome 1. (B) Several probes, each corresponding to a defined genomic ...

All steps of ISH – from pre-hybridization to additions of probes, hybridization, stringency washes and antibody-mediated chromogenic or fluorescent reaction – are carried out by the robotic system.