and probe-based (for example, hydrolysis probes). SYBR Green–based assays are considered very flexible as they only require the design of a primer pair, but they are generally less specific than ...

BHQplus Probes form more stable probe-target duplexes, enabling shorter oligonucleotide designs. Shorter oligos offer high specificity and better detection of difficult targets, such as AT-rich ...

LGC Biosearch Technologies offers high-quality and cost-effective MGB Probes. These dual-labeled hydrolysis probes consist of a 5' fluorescent reporter dye and a 3' Eclipse Dark Quencher (EDQ ...

RT-qPCR can be performed using various fluorescent chemistries (e.g., DNA binding dyes, TaqMan® hydrolysis probes [Roche], Lightcycler® dual hybridization probes [Roche], Molecular Beacons ...