The assay is run in two main steps, the kinase reaction step followed by the detection step with HTRF reagents (Fig. 1). The phosphorylation of the biotin-tagged generic peptide is initiated by ...

Glycogen synthase kinase 3 (GSK3) was initially described ... Figure 4: The molecular mechanism by which phosphorylation inhibits GSK3. Figure 5: Several signalling pathways induce the inhibition ...

RNA cleavage can occur either: (i) by a transesterification mechanism that yields 2’,3’-cyclic-PO 4 and 5’-OH ends ... The Aspergillus, Coccidioides, and Candida Trl1 enzymes all strongly prefer GTP ...