I therefore devised a single-tube, nested PCR method that is easy to handle and yields consistent results. The PCR mix contained 8 pmol of each reverse and FAM-M13(-21) primer and 2 pmol of the ...

You have full access to this article via your institution. To demonstrate the power of PCR–mediated recombination and provide an example of the general utility of the method, we constructed ...

This infographic breaks down the technology behind each technique, as well as their workflows, applications and important the ...

Here’s a handy overview of the technique, how it works and a few refresher details on viruses and genetics. What is real time RT–PCR? Real time RT–PCR is a nuclear-derived method for detecting the ...

Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount of DNA present in a sample. The procedure requires specialized thermal cyclers with ...

Aside from producing a result far faster than a typical polymerase chain reaction (PCR) test, the procedure costs about the same, just $2 (269 yen). Team members relied on technology developed ...