All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

and to order the forward primers so that the M13(-21) sequence is appended at its 5′ end. The advantage of the one-time order is that one can choose the best and more expensive energy transfer ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...