The amount of input target protein sample to use depends on the detection limit of the assay, but 10% of the amount added to each binding reaction (3 μl) is usually sufficient. The unbound target ...
For this reason, the version of the NoShift Assay Kit used here includes components (the NucBuster Protein Extraction Kit) that yield a nuclear extract suitable for DNA binding studies in less ...
More information: Kejia Li et al, A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands, Nature Methods (2024).