Each TaqMan probe consists of a single-stranded oligonucleotide that is complementary to a sequence within the target template. It has a fluorescent dye at its 5′ end, whose signal is stifled by ...

A major advantage of drop-off digital PCR is the single-assay detection ... The simplest version of a drop-off assay includes two TaqMan™ probes targeting the same amplicon: a drop-off probe ...

Users can easily transition from a present TaqMan assay to a TaqPath assay or enter their primer and probe sequences for a custom TaqPath experience. Provide sequences for forward and reverse primers.

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

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Both the Mustang Purple TM passive reference dye-containing mix and the NO ROX mix can be used with TaqMan probes that contain ... master mixes have 72-hour pre-PCR benchtop stability.

This innovative kit allows for the rapid detection of bacterial and fungal contamination within a mere 3 hours, leveraging the accuracy and reliability of real-time PCR technology ... High Specificity ...

Total RNA (1pg, 100pg, 10pg, 1pg) was purified from a breast tissue FFPE sample using the RNeasy FFPE Kit. Transcripts of the human MUC1 gene were amplified and detected using the QuantiFast Probe ...

Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages ...