Inoculate one tube of SIM medium using an inoculating needle to make a single stab to the bottom of the tube. _____ Inoculate one slant (Basic Slant Inoculation Procedure) of Simmons Citrate Agar ...

The first selective ingredient in this agar is bile, which inhibits the growth of Gram-positives other than ... Esculetin reacts with ferric citrate (in the medium), forming a phenolic iron complex ...