实验结果显示，S1核酸酶的存在导致了Q-ssDNA-FAM连接体显著的 ... 以改进AIEgen报告基因，与Class 2 Crispr/Cas系统（Cas12和Cas13）结合用于直接检测DNA和RNA ...

物理隔离是实现”一管式”CRISPR检测的最直接方法，常见的方式是通过特制的隔离介质将扩增和酶切反应固定于两个腔室中，扩增后再通过手动操作将酶切体系和扩增产物混合，但这种方式仍面临试剂固定稳定性、操作复杂等挑战。微流控芯片通过集成多个反应室于单一反应 ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

The purified crRNA was used in the CRISPR/Cas12a reaction. The 20 μL reaction mixture comprised 2 μL of 10× HOLMES Buffer 1 (Tolobio, China), 0.5 μL of 10 μM Cas12a protein (EZassay Biotech, China), 0 ...

Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States Center for Synthetic Biology, Northwestern University, Evanston, Illinois 60208, ...

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CRISPR stocks are high risk but hold potential for aggressive investors. CRISPR Therapeutics and its partner Vertex Pharmaceuticals have the only approved CRISPR therapy. Several other biotech ...

Do you actually know how to use the crisper drawer in the refrigerator, or do you find yourself cramming produce in there in a mad dash to unload the groceries? I'll be the first one to admit it ...