In the current installment, [Justin] covers the basics of agarose gel ... DNA stain for visualizing the bands; we’re heartened to see that the potent carcinogen ethidium bromide that we used ...
without ethidium bromide and allow the gel to set. Run the gel in the same buffer solution as used for preparing the gel. 2 | Prepare agarose plugs containing the DNA of interest and carry out ...
Optionally, the gel may be tested for complete transfer of the DNA by staining in 0.5 µg/ml ethidium bromide and viewing under UV light. 2. Cut gel in half between the 2 sets of lanes. Each group will ...
Protein gels stained with Coomassie Blue or silver, as well as fluorescent ethidium bromide or SYBR Safe DNA gels, can be easily imaged using the system's GeneSys software, which also selects the ...
The use of SYBR ® Safe DNA Gel Stain and a Safe Imagerâ„¢ Transilluminator ensures maximum possible safety to the user and gives improved cloning efficiency over ethidium bromide and UV.