PCR-Machines are classified depending on the capacity of PCR used such as Nested PCR or Reverse Transcriptase PCR (RT-PCR ), or Multiplex RT PCR. PCR-machines are used for replication of DNA ...
A negative control was prepared using sterilized water as a template. The step program for PCR was as follows: 95 °C for 30 s, followed by 40 cycles of 95 °C for 10 s (denaturation) and 60 °C for 30 s ...
I therefore devised a single-tube, nested PCR method that is easy to handle and yields consistent results. The PCR mix contained 8 pmol of each reverse and FAM-M13(-21) primer and 2 pmol of the ...
A nested PCR approach was chosen as it produced a cleaner endpoint PCR product than a one-step PCR with an increased number of cycles. Quantitation of the endpoint PCR products by solid-phase ...
% how to perform an statistical analysis of a group of bipartite networks % (matrices). For doing that we will use the data from Flores et Al 2011. % This data consist of 38 bipartite adjacency ...
Serotyping, PCR, phage-typing and antibiotic sensitivity testing of Salmonella serovars isolated from urban drinking water supply systems of Nepal. Polyurethane foam as an inert carrier for the ...
The researchers adapted a bacteriophage-based method combined with PCR (phage-PCR) and using a new isothermal DNA amplification protocol using Recombinase Polymerase Amplification (RPA) to detect and ...
Qingdao Key Laboratory of Materials for Tissue Repair and Rehabilitation, School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao 266024, China ...
Introduction: The development of a hepatitis E virus (HEV) vaccine is critical, with ORF2 capsid protein as the main target. We previously demonstrated that oral coadministration of recombinant ORF2 ...
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