Frontiers | An Overview of Methods and Tools for Transfection of ...
2021年7月19日 · Transfection is a widely used laboratory cell culture technique that introduces foreign nucleic acids into cells. It is a powerful analytical tool enabling study of gene functions and gene products in cells.
Transfection types, methods and strategies: a technical review - PeerJ
2020年7月22日 · In this review, we summarized and compared the findings from various reported literature on the characteristics, strengths, and limitations of various transfection methods, type of transfected nucleic acids, transfection controls and approaches to assess transfection efficiency.
Mammalian cell transfection: the present and the future - PMC
Transfection is a procedure that introduces foreign nucleic acids into cells to produce genetically modified cells. Transfection is a powerful analytical tool for study of gene function and regulation and protein function.
Transfection types, methods and strategies: a technical review
2021年4月21日 · Transfection is a modern and powerful method used to insert foreign nucleic acids into eukaryotic cells. The ability to modify host cells' genetic content enables the broad application of this process in studying normal cellular processes, disease molecular mechanism and gene therapeutic effect.
Transfection types, methods and strategies: A technical review
2021年4月21日 · In this review, we summarized and compared the findings from various reported literature on the characteristics, strengths, and limitations of various transfection methods, type of transfected...
Transfection is a modern and powerful method used to insert foreign nucleic acids into eukaryotic cells. The ability to modify host cells’ genetic content enables the broad application of this process in studying normal cellular processes, disease molecular mechanism and gene therapeutic effect.
Transfection reflections: fit-for-purpose delivery of nucleic acids ...
2023年6月21日 · Transfection is a foundational technology in biosciences, biotechnology, and the development of biotherapeutic agents. Given its transcendence, it is problematic that transfection remains a...
Comparing chemical transfection, electroporation, and lentiviral …
2024年5月13日 · Transfection is an important analytical method for studying gene expression in the cellular environment. There are some barriers to efficient DNA transfection in host cells, including circumventing the plasma membrane, escaping endosomal compartmentalization, autophagy, immune sensing pathways, and translocating the nuclear envelope.
Review Transient and stable vector transfection: Pitfalls, off …
2017年7月1日 · In this review, we demonstrate that an assumption that empty vector-transfected cells preserve the cytogenetic and phenotypic characteristics, and represent the adequate control in transfection experiments is not universally valid.
Conceptual and technical aspects of transfection and gene delivery
2015年3月15日 · Here we give an overview of established and emerging methods of transfection and gene delivery and provide conceptual and technical advantages and drawbacks of their particular use. Additionally, based on a flow chart, we compiled a rough guideline to choose a gene transfer method for a particular field of application.