RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [ 2 ]

Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.

Choose RPMI 1640 Media in standard, innovative, or custom formulations to fit your cell culture needs. RPMI 1640 is vitamin-rich and contains the reducing agent glutathione.

Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

DMEM is a nutrient-rich medium that supports the growth of a wide range of cell types, making it suitable for general cell culture applications. On the other hand, RPMI is specifically designed for the growth of immune cells and is often used in immunology and cancer research.

RPMI 1640 Medium is the only media that contains the reducing agent glutathione and high concentrations of vitamins and choline. It also contains vitamin B12, biotin, and PABA, which are not found in DMEM.

RPMI-1640 medium is commonly used for suspension cultures of mammalian and primary cells, such as HeLa cells, human peripheral blood mononuclear cells (PBMCs), human normal and neoplastic leukocytes, Jukrat cells, and more.

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. With 2,000 mg/L D-glucose, non-essential amino acids (NEAA), and 110 mg/L sodium pyruvate.

Supports cell growth in research and biomanufacturing cell culture processes. HyClone RPMI 1640 medium is extensively quality control tested to ensure lot-to-lot consistency. Medium efficacy and homogeneity of RPMI 1640 is tested with HyClone sera. HyClone RPMI solution with L-glutamine is available with or without HEPES.

RPMI 1640 contains a balanced mixture of salts, vitamins, amino acids, and glucose, which supports optimal cell growth. The medium is particularly effective for culturing human leukemic cells and is often supplemented with serum to enhance its performance.