PCR Amplification | An Introduction to PCR Methods | Promega
However, PCR has evolved far beyond simple amplification and detection, and many extensions of the original PCR method have been described. This chapter provides an overview of …
PCR | PCR kits | Polymerase Chain Reaction - Promega Corporation
From endpoint PCR to quantitative real-time PCR, and from hot-start enzymes and master mixes to dNTPs and buffers, we offer a complete portfolio of high-quality PCR products to meet your …
STR Amplification | STR Analysis | STR kits - Promega Corporation
Promega offers STR amplification systems for forensic, paternity and relationship-testing applications that meet global and regional database requirements. PowerPlex® Systems are …
Introduction to PCR Animation - Promega Corporation
Learn the fundamental concept of the polymerase chain reaction (PCR), a landmark molecular biology technique. Don't miss out! Stay notified of Promega events, products and news.
Scientific Issues with Analysis of Low Amounts of DNA - Promega …
Stochastic (random) variation is a fundamental physical law of the PCR amplification process when examining low amounts of DNA. Stochastic effects are manifest as a fluctuation of …
The PCR Method for the Maxprep™ Liquid Handler is designed to automate preparation of endpoint and quantitative PCR and RT-PCR amplifications, and includes master mix …
I. Real-Time PCR overview •Basics of Real-Time PCR •Understanding the data II. Chemistries and Instrumentation •Dye-based chemistries (e.g. SYBR) •Label-based chemistries (e.g. …
Nucleic Acid Amplification - Promega
After you've purified and quantitated your nucleic acid, the next step is amplification. PCR is perhaps the most ubiquitous assay in molecular biology—something we do every day. Each …
qPCR and RT-qPCR - worldwide.promega.com
The primary output from your real-time PCR instrument is an amplification curve. The curve shows the normalized fluorescent signal versus the cycle number and thus shows the …
Understanding the IPC in DNA Quantitation Assays - Promega …
Many real-time quantitative PCR assays like the Plexor® HY System include an internal PCR control (IPC) to test for the presence of PCR inhibitors in the DNA samples.