A guide to protein purification technologies, including affinity purification tags such as GST, His-tag, HaloTag and biotin, as well as pull-down systems for purification of protein complexes.

A guide to protein purification technologies, including affinity purification tags such as GST, His-tag, HaloTag and biotin, as well as pull-down systems for purification of protein complexes.

Promega’s unique portfolio of protein analysis laboratory products include mass spec proteases, protein purification tags/resins and fluorescent ligands, cell-free and cell-based protein expression systems, and novel methods for detecting and studying proteins at endogenous expression levels.

Affinity tags are a selective and versatile way to purify your protein of interest. Use this introductory guide to learn about what tagging systems are available and how they work.

Whether you need an affinity tag for protein purification, want to create a single fusion protein to be used in a variety of protein analysis methods, or add an endogenous tag using CRISPR-Cas9 gene editing, there are an array of tags and reporter enzymes available.

The MagneGSTTM Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins with high efficiency and low background.

HaloTag® Protein Purification Systems provide a rapid, reliable method for purification of proteins expressed in E. coli and mammalian cells. For E. coli, the HaloTag® protein tag enhances expression and solubility of recombinant proteins.

An introduction to protein expression. Covers the basics of in vitro transcription and translation and cell-free protein expression methods. Protocols for basic to advanced cell-free expression systems are provided.

The HQ-tagged proteins can be purified using multiple formats such as: 1) small-scale purification using the MagneHisTM Ni-Particles; 2) small-scale vacuum-based format using the HisLinkTM 96 Protein Purification System (Cat.#

Promega has performed a thorough investigation of methods at different points in the purification process to ensure the isolation of high-quality DNA from EndA + (wildtype) bacterial strains.