U6 snRNA is the non-coding small nuclear RNA (snRNA) component of U6 snRNP (small nuclear ribonucleoprotein), an RNA-protein complex that combines with other snRNPs, unmodified pre-mRNA, and various other proteins to assemble a spliceosome, a large RNA-protein molecular complex that catalyzes the excision of introns from pre-mRNA.

Short untranslated RNAs such as the entire pool of transfer RNAs (tRNAs), the 5S rRNA and the U6 spliceosomal RNA (snRNA), which are critical for cellular function, are transcribed by RNAPIII. Assembly of the molecular machinery that initiates RNAPIII-dependent transcription falls into three main classes corresponding to three different types ...

2020年6月20日 · U6 folds with U2 snRNA into an RNA-based active site that positions the 5'SS at two catalytic metal ions. The branch point (BP) adenosine attacks the 5'SS, producing a free 5' exon. Removal of the BP adenosine from the active site allows the 3'SS to bind, so that the 5' exon attacks the 3'SS to produce mature mRNA and an excised lariat intron.

U6 snRNA is one of five uridine-rich noncoding RNAs that form the major spliceosome complex. Unlike other U-snRNAs, it reveals many distinctive aspects of biogenesis such as transcription by RNA polymerase III, transcript nuclear retention and particular features of transcript ends: monomethylated 5 …

2024年10月1日 · Considering ILP1 and NTR1 interacts with spliceosome-associated proteins to promote ILS disassembly (Wang et al., 2019), we analyzed the physical relationship between these two proteins and U6 snRNA to gain deeper insights into the mechanism by which ILP1 and NTR1 proteins influence U6 stability. RNA immunoprecipitation analysis was conducted ...

2021年5月28日 · N6 -methyladenosine (m 6 A) is a modification that plays pivotal roles in RNA metabolism and function, although its functions in spliceosomal U6 snRNA remain unknown. To elucidate its...

2001年6月1日 · The major spliceosomal UsnRNPs U1, U2, U4, U5 and U6 are responsible for splicing the vast majority of pre-mRNA introns (so-called U2-type introns). A group of less abundant snRNPs, U11, U12, U4atac and U6atac, together with U5, are subunits of the so-called minor spliceosome that splices a rare class of pre-mRNA introns, denoted U12-type [3 ...

Decades of biochemical and genetic data, along with recent cryo-EM structures, unequivocally demonstrate that U6 snRNA forms much of the catalytic core of the spliceosome and is highly dynamic, interacting with three snRNAs, the pre-mRNA substrate, and >25 protein partners throughout the splicing cycle.

3 天之前 · According to El Fatimy et al. , miR-10b, an oncomiR associated with malignant gliomas, was demonstrated to bind U6 snRNA, a crucial part of the spliceosomal machinery, representing an unexpected ...

Decades of biochemical and genetic data, along with recent cryo-EM structures, unequivocally demonstrate that U6 snRNA forms much of the catalytic core of the spliceosome and is highly dynamic, interacting with three snRNAs, the pre-mRNA substrate, and >25 protein partners throughout the splicing cycle.