Assembly of Outer Membrane β-Barrel Proteins: the Bam Complex
The BamA protein is the most highly conserved member of the Bam complex across Gram-negative genera (Table 1). Indeed, the BamA protein has predicted functional and structural homologs in the OMs of chloroplasts (48, 75, 78) and in mitochondria (59, 111, 142).
P0A940 - UniProt
2005年7月19日 · Part of the outer membrane protein assembly complex (Bam), which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamD, the core component of the assembly machinery.
Bacterial outer membrane proteins assemble via asymmetric
2019年7月26日 · Here authors develop a method to trap an E. coli β-barrel protein bound stably to BamA at a late stage of assembly in vivo which provides insights BamA mediated membrane integration.
Structural insight into the biogenesis of β-barrel membrane proteins
2013年9月1日 · β-barrel membrane proteins are essential for nutrient import, signalling, motility and survival. In Gram-negative bacteria, the β-barrel assembly machinery (BAM) complex is...
The assembly of β-barrel outer membrane proteins - PMC
The five-protein β-barrel assembly machine (Bam) accelerates the folding and membrane integration of these proteins. The central component of the machine, BamA, contains a β-barrel domain that can adopt a lateral-open state with its N- and C-terminal β-strands unpaired.
Mutational and Topological Analysis of the Escherichia coli BamA Protein
To gain insight into the workings of the BAM complex we have used genetic, biochemical and modeling approaches to identify important functional and structural regions of the E. coli BamA protein and have developed a topological prediction of the BamA β-barrel.
Immunoprotective potential of BamA, the outer membrane protein …
2017年9月29日 · BamA, an outer membrane β-barrel assembly protein, was identified in A. baumannii as potential vaccine candidate by in silico analysis. The immunoprotective efficacy of this highly conserved protein was investigated against a virulent multidrug resistant clinical isolate using murine pneumonia model.
Inhibitors targeting BamA in gram-negative bacteria☆
2024年1月1日 · The outer membrane, a unique and essential structure in gram-negative bacteria, contains multiple potential antibacterial targets including BamA, an outer membrane protein that folds and inserts transmembrane β-barrel proteins.
Formation of a β-barrel membrane protein is catalyzed by the ... - eLife
2019年11月14日 · BamA, an essential component of the complex, is itself a β-barrel and is proposed to play a central role in assembling other barrel substrates. Here, we map the path of substrate insertion by the Bam complex using site-specific crosslinking to understand the molecular mechanisms that control β-barrel folding and release.
In vivo roles of BamA, BamB and BamD in the biogenesis of BamA…
By employing a BamA protein bearing one such substitution, F494L, it was demonstrated that the mutant BamA protein could not only assemble without BamD, but it could also facilitate the assembly of wild-type BamA expressed in trans. Based on these data, we propose a model in which the Bam lipoproteins, which are localized to the outer membrane ...
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