Cyanine - Wikipedia
Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. Cy3 and Cy5 are the most popular, typically used combined for 2 color detection.
Sequence-dependence of Cy3 and Cy5 dyes in 3ʹ terminally ...
2022年8月31日 · In this paper, we comprehensively map the sequence-dependence of Cy3 and Cy5 dyes in 3ʹ-fluorescently labeled single-stranded DNA by preparing the complete permutation library of the 5...
What is the difference between Cy3 and Cy5? – SyronOptics
Cy3 and Cy5 are both fluorescent dyes belonging to the cyanine dye family, widely used in molecular biology, biotechnology, and medical diagnostics for labeling nucleic acids, proteins, and other biological molecules.
Mechanism of Cyanine5 to Cyanine3 Photoconversion and Its ...
2021年8月25日 · Indocarbocyanine dyes, such as Cyanine3 (Cy3), Cyanine5 (Cy5), Cyanine7 (Cy7), and Alexa Fluor 647 (AF647), are popular choices for fluorescence-based applications due to their high molar extinction coefficients and tunability of fluorescence wavelengths as well as commercial availability, including their derivatives designed for target ...
Sequence-Dependent Fluorescence of Cy3- and Cy5-Labeled ...
2016年2月19日 · The cyanine dyes Cy3 and Cy5 are among the most widely used and versatile oligonucleotide labels in, e.g., microarray experiments, fluorescent in situ hybridization (FISH), real-time PCR (RT-PCR), and FRET studies and are considered to …
In this paper, we comprehensively map the sequence‐dependence of Cy3 and Cy5 dyes in 3ʹ‐fluorescently labeled single‐stranded DNA by preparing the complete permutation library of the 5...
What is the difference between Cyanine 3 and Cyanine 5?
While Cy3 and Cy5 share similarities as cyanine dyes used for fluorescence labeling, they have distinct absorption and emission wavelengths that make them suitable for different types of fluorescence-based applications.