Ellman's reagent (5,5′-dithiobis- (2-nitrobenzoic acid) or DTNB) is a colorogenic chemical used to quantify the number or concentration of thiol groups in a sample. [3] . It was developed by George L. Ellman.

Thermo Scientific Pierce Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.

DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.

5,5′-Dithiobis (2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. [1] . It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. [2] . Thiolate anion (RS -) reacts with Ellman′s reagent (DTNB 2-), to produce 2-nitro-5-thiobenzoate anion (TNB 2-) and one mixed disulfide (R-S-TNB -).

DTNB has little if any absorbance, but when it reacts with -SH groups on proteins under mild alkaline conditions (pH 7-8), the 2-nitro-5-thiobenzoate anion (TNB2-) gives an intense yellow color at 412 nm.3. The optimal molar extinction coefficient value for …

Overcome challenges in synthesis and disulfide bond formation with protocols for Fmoc solid-phase peptide synthesis of peptides with cysteine and methionine. DTNB, or Ellman′s …

Ellman’s reagent is 5,5’-dithio-bis- (2-nitrobenzoic acid), also known as DTNB, a compound used for quantitating free sulfhydryl groups in solution (Fig. 1). A solution of this compound produces a yellow-colored product when it reacts with sulfhydryl groups.

Ellman’s reagent 5,5′ -dithiobis (2-nitrobenzoic acid) (DTNB) was first introduced in 1959 for the estimation of free thiol groups (1). The procedure is based on the reaction of the thiol with DTNB to give the mixed disulfide and 2-nitro-5-thiobenzoic acid (TNB) which is quantified by the absorbance of the anion (TNB 2-) at 412 nm.

Check with your regional distributor for final pricing. Ellman"s Reagent or DTNB, this was originally developed for use as a sulfhydryl reagent. It can be used to quantitate thiols in proteins, cells and plasma by absorption measurements.

DTNB reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (TNB; see Figure 2). The target of DTNB in this reaction is the conjugate base (R—S-) of a free sulfhydryl group.