Electrophoretic mobility shift assay - Wikipedia
An antibody that recognizes the protein can be added to this mixture to create an even larger complex with a greater shift. This method is referred to as a supershift assay, and is used to unambiguously identify a protein present in the protein – nucleic acid complex.
Electrophoretic Mobility Shift Assay (EMSA) for Detecting …
The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids. It is the core technology underlying a wide range of qualitative and quantitative analyses for the characterization of interacting systems.
Gel Shift Assays (EMSA) | Thermo Fisher Scientific - US
This article provides information about studying DNA–protein interactions using an electrophoretic mobility shift assay (EMSA).
Electrophoretic mobility shift assay (EMSA) for detecting …
2007年7月26日 · The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids.
Electrophoretic Mobility Shift Assay (EMSA): Principle, …
2023年4月18日 · Electrophoretic Mobility Shift Assay (EMSA) is a biochemical method used to examine how proteins interact with nucleic acids like DNA or RNA as well as DNA-RNA interactions. It is also known as a band shift assay or a gel shift test.
Electrophoretic mobility shift assays | Nature Methods
The electrophoretic mobility shift assay (EMSA), one of the most sensitive methods for studying the DNA-binding properties of a protein, can be used to deduce the binding parameters and...
An Optimized Protocol for Electrophoretic Mobility Shift Assay …
Electrophoretic Mobility Shift Assays (EMSA) are an instrumental tool to characterize the interactions between proteins and their target DNA sequences. Radioactivity has been the predominant method of DNA labeling in EMSAs.
What is an Electrophoretic Mobility Shift Assay? - News-Medical.net
2020年9月29日 · Electrophoretic mobility shift assay, sometimes referred to as EMSA, is a sensitive method for investigating nucleic acid-protein binding.
Electrophoretic Mobility Shift Assay - an overview - ScienceDirect
The electrophoretic mobility shift assay (EMSA) technique allows the in vitro detection of protein–DNA binding and is based on the observation that a protein–DNA complex migrates more slowly than the free DNA when subjected to gel electrophoresis.
Electrophoretic Mobility Shift Assay - an overview - ScienceDirect
Electrophoretic Mobility Shift Assay is a technique used to study protein-DNA interactions by observing the movement of DNA-protein complexes in gel electrophoresis, where the complex moves slower than free DNA.