Real time imaging of single extracellular vesicle pH ... - Nature
2022年1月10日 · Extracellular vesicles (EVs) are cell-derived membranous structures carrying transmembrane proteins and luminal cargo. Their complex cargo requires pH stability in EVs while traversing diverse...
Effect of pH, temperature and freezing-thawing on quantity changes …
2018年4月3日 · Some reports revealed that the acidic pH could reduce degradation of exosome-associated proteins and higher yield of exosome could be isolated after adjustment of pH value to under 7 in conditioned medium or urine and incubation at RT for …
Exosome Release and Low pH Belong to a Framework of …
2014年2月6日 · The results showed that CisPt uptake by human tumour cells was markedly impaired by low pH conditions. Moreover, exosomes purified from supernatants of these cell cultures contained various amounts of CisPt, which correlated to the pH conditions of …
Environmental pH stress influences cellular secretion and uptake …
2021年2月18日 · Exosomes (extracellular vesicles/EVs) participate in cell–cell communication and contain bioactive molecules, such as microRNAs. However, the detailed characteristics of secreted EVs produced by cells grown under low pH conditions are still unknown.
Effect of pH, temperature and freezing-thawing on quantity
Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes Protein Cell . 2019 Apr;10(4):295-299. doi: 10.1007/s13238-018-0529-4.
pH-Mediated Clustering of Exosomes: Breaking Through the Size …
2021年10月5日 · Here, we report a pH-mediated assembly system that converts single nanosized exosomes into microsized clusters, which can be directly analyzed by conventional flow cytometry (FCM), breaking through the size limit of exosome analysis.
Microenvironmental pH and Exosome Levels Interplay in Human ... - PubMed
2018年10月5日 · The results showed that pH 6.5 induced a remarkable increase in exosome release, and buffering the medium significantly reduced the exosome release in all cancers. With these results, we provide, for the first time, evidence that tumor acidity and exosome levels represent common cancer phenotypes.
A live cell reporter of exosome secretion and uptake reveals ...
2020年4月29日 · By making a single amino acid mutation, we developed a stable and bright pH-sensitive reporter, pHluo_M153R-CD63, for live imaging of MVB fusion and cellular interactions with extracellular...
Low pH increases the yield of exosome isolation - PubMed
2015年5月22日 · In this research, we investigated the yield of total exosomal protein and RNA after incubation in acidic, neutral and alkaline conditioned medium. Representative exosome markers were investigated by western blot after incubation of exosomes in different pH.
Microenvironmental pH and Exosome Levels Interplay in Human …
The results showed that pH 6.5 induced a remarkable increase in exosome release, and buffering the medium significantly reduced the exosome release in all cancers. With these results, we provide, for the first time, evidence that tumor acidity and exosome levels represent common cancer phenotypes.
- 某些结果已被删除