A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
2022年7月21日 · Fluorescence lifetime imaging microscopy (FLIM) exploits the inherent properties of fluorescent dyes to produce an image. Fluorescent molecules have become essential tools for cell biologists. They are widely used in microscopy to visualize many different structures, targets and dynamic processes within cells, tissues, and even whole organisms.
An Introduction to Fluorescence | Science Lab - Leica Microsystems
2023年6月2日 · For microscopy, fluorescence is the most useful kind of luminescence. Fluorochromes can easily be excited with their specific wavelength via certain light sources, e.g., lamps and filter systems or lasers, and the emitted light can be distinguished from the excitation light by the wavelength (Stokes’ shift).
Fluorescence | Applications - Leica Microsystems
Find out how fluorescence microscopes from Leica Microsystems support your research. Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its high sensitivity and high specificity. Fluorescence is a form of luminescence that through microscopy allows users to determine the distribution of a single molecule species, its …
Fluorescence in Microscopy | Science Lab | Leica Microsystems
2011年4月27日 · Fluorescence microscopy is a special form of light microscopy. It uses the ability of fluorochromes to emit light after being excited with light of a certain wavelength. Proteins of interest can be marked with such fluorochromes via antibody staining or …
Introduction to Widefield Microscopy - Leica Microsystems
2017年6月29日 · The main advantages of confocal microscopy are that user-defined regions of interest can be selected, negating the need for the entire specimen to be exposed to a fluorescent light source. In addition, the confocal microscope can be used to obtain optical sections through a specimen which can have the advantage of excluding much of the out-of ...
Fluorescent Dyes | Science Lab | Leica Microsystems
2022年1月10日 · A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP – genetically linked to the protein of interest. If cloning is impossible – for instance in histologic samples – techniques such as immunofluorescence staining are used to visualize …
What is FLIM - Fluorescence Lifetime Imaging Microscopy?
2022年11月15日 · FLIM exploits fluorescence lifetime which is a measure of how long a fluorescent molecule or fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon. The emission of a fluorescence photon from a fluorophore does not always occur at an exact time after excitation. Instead, a distribution of …
The Fundamentals and History of Fluorescence and Quantum Dots
2017年11月15日 · At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and trace anything from whole organisms to single proteins and beyond. In this article, we will examine what is meant by "fluorescence", the history and basic …
Epi-Illumination Fluorescence and Reflection-Contrast Microscopy ...
2023年11月2日 · Compared to today, Fluorescence microscopy was applied for the first time with the use of transmitted light and darkfield microscopy, due to its limited range of applications at that time. But with its increasing importance for histology, cytology, molecular biology, and immuno-diagnosis, the demand for a fundamental improvement of illumination ...
How to Prepare your Specimen for Immunofluorescence Microscopy
2022年1月10日 · Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM, Epifluorescence, TIRF, GSDIM), depending on the application or the researcher’s interest. Meanwhile, IF has become indispensable for a large number of research …