Lipofectamine™ 3000 Transfection Reagent - Thermo Fisher Scientific
Lipofectamine 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.
Lipofectamine 3000 Transfection Reagent - Thermo Fisher Scientific
Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum.
The intracellular trafficking mechanism of Lipofectamine …
2016年5月11日 · We demonstrate here that Brownian diffusion is an efficient route for Lipofectamine/DNA complexes to avoid metabolic degradation, thus leading to optimal transfection. By contrast, active...
Liposome-Mediated Transfection: Mechanisms, Protocols, and FAQs
Lipofection is a form of non-viral transfection that employs liposomes - synthetic lipid bilayers that can encapsulate nucleic acids like plasmid DNA, mRNA, or siRNA. Liposomes are used as a tool for the purpose of in vivo or in vitro delivery of carriers.
Lipofectamine 2000™ at transfection dose promotes EphA2 …
In this study, we found that Lipofectamine™ 2000 (lipo2000), a commonly used commercial cationic liposome transfection, could upregulate EphA2 mRNA expression in multiple cells at transfection dose. Furthermore, lipo2000 treatment could increase the level of EphA2 hnRNA (heterogeneous nuclear RNA).
Make sufficient 10% FBS in Opti-MEM media (w/o antibiotics) sterile filter.
Advanced transfection with Lipofectamine 2000 reagent: primary neurons ...
2004年6月1日 · The importance of these factors in Lipofectamine 2000 mediated transfection will be discussed together with some specific applications: transfection of primary neurons, high throughput transfection, and delivery of small interfering RNAs.
Lipofection - an overview | ScienceDirect Topics
Lipofection, also known as “lipid transfection” or “liposome-based transfection,” uses a lipid complex to deliver DNA to cells. From: Guide to Research Techniques in Neuroscience (Second Edition), 2015
InvitrogenTM LipofectamineTM 3000 Transfection Reagent, a reagent developed to improve delivery and enable use of new technologies, can be used in more relevant systems and enables faster and more reliable outcomes.
With transfection success across multiple cell lines, Lipofectamine™ 2000 gives you the most versatile route to efficient gene delivery and protein expression (Table 1).
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