Molecular beacon - Wikipedia
Molecular beacons, or molecular beacon probes, are oligonucleotide hybridization probes that can report the presence of specific nucleic acids in homogenous solutions. Molecular beacons are hairpin -shaped molecules with an internally quenched fluorophore whose fluorescence is restored when they bind to a target nucleic acid sequence.
molecular beacons
2025年1月8日 · Molecular beacons are single-stranded oligonucleotide hybridization probes that form a stem-and-loop structure. The loop contains a probe sequence that is complementary to a target sequence, and the stem is formed by the annealing of complementary arm sequences that are located on either side of the probe sequence.
We call these probes "molecular beacons" because they emit a fluores cent signal only when hybridized to target molecules. Since unhy bridized molecular beacons are dark, it is not necessary to...
Molecular beacon probes - Integrated DNA Technologies
Molecular beacon probes are dual-labeled probes that form a quenched, stem-loop structure in native state and fluoresce upon hybridization to the target nucleotide sequence. Applications include real-time and endpoint PCR, SNP detection, and multiplex amplification.
Molecular Beacons - MilliporeSigma
A Molecular Beacon is a single-stranded bi-labeled fluorescent probe held in a hairpin-loop conformation (20 to 25 nt is most common) by complementary stem sequences (around 4 to 6 nt) at both ends of the probe.
Application of Molecular Beacons in Real-Time PCR - PMC
The molecular beacons are the ideal hybridization-based probe for short oligonucleotide detection, and, thus, it is a suitable probe for real-time PCR . In real-time PCR, MB hybridizes with template DNA at the annealing step and produces the fluorescent signal directly.
Molecular Beacon - an overview | ScienceDirect Topics
Molecular beacons are nucleic acid hybridization probes. They are designed to bind to target DNA sequences in regions, such as the rpoB, where resistance mutations are known to occur. Molecular beacons will fluoresce only when bound to their targets so that a mutation – even a single nucleotide substitution – will prevent fluorescence.
Molecular Beacons - MilliporeSigma
Molecular Beacon probes fluoresce upon hybridization with a complementary target sequence. These structured probes are highly sensitive, sequence specific, and are used for sequence detection in qPCR for in vitro studies.
Molecular Beacons: A Novel Optical Diagnostic Tool - PMC
Molecular beacons (MBs) are single-stranded, fluorophore-labeled nucleic acid probes that are capable of generating a fluorescent signal in the presence of target, but are dark in the absence of target.
Molecular Engineering of DNA: Molecular Beacons - PMC
Molecular beacons (MBs) are specifically designed DNA hairpin structures that are widely used as fluorescent probes. Applications of MBs range from genetic screening, biosensor development, biochip construction, and the detection of single-nucleotide polymorphisms to mRNA monitoring in …