Vivid COVID-19 LAMP is an ultrasensitive, quadruplexed test
2023年3月2日 · We selected the envelope (E) gene as our first target to design LAMP primers because it is conserved, located towards the 3’ end of the SARS-CoV-2 genome, and its transcripts are enriched in...
Development of loop-mediated isothermal amplification (LAMP) …
2023年3月28日 · Results showed that the five-primer design provides stable primer targeting and avoids misamplification for 120 min. Optimizing the colorimetric and fluorometric RT-LAMP assays decreased...
Improving RT-LAMP detection of SARS-CoV-2 RNA through primer …
Typical RT-LAMP reactions use a primer set covering 8 regions on the target sequence and the primer design is facilitated by software (Primer Explorer V5 at Eiken https://primerexplorer.jp/e/, or LAMP Primer Design Tool at NEB https://lamp.neb.com/#!/) based on oligo length, AT and GC content and thermodynamic stability. However, like primers ...
An Innovative AI-based primer design tool for precise and …
2023年9月22日 · The samples are tested with DeCoV19 Kit Triplex, GeneFinder COVID-19 PLUS RealAmp Kit, and the AI-designed primers UtrechtU-ORF3a. Then, viral RNA is extracted from nasopharyngeal and ...
Developing RT-LAMP assays for rapid diagnosis of SARS-CoV-2 …
2021年7月1日 · In this study, we developed an improved bioinformatics algorithm to design LAMP primer sets targeting the nucleocapsid (N), membrane (M), and spike (S) genes of SARS-CoV-2. The performance of these primer sets was rigorously validated experimentally.
Development of a multiplex Loop-Mediated Isothermal Amplification (LAMP ...
2021年3月3日 · In this study, we have designed three reverse-transcription loop-mediated isothermal amplification (RT-LAMP) primer sets to detect the RNA-dependent RNA polymerase (RdRP), Envelope (E) and Nucleocapsid protein (N) genes of SARS CoV-2.
LAMPrimers iQ: New primer design software for loop-mediated
2023年11月2日 · Here, we report on a new program called LAMPrimers iQ destined for high-quality LAMP primers design. LAMPrimers iQ is based on an original algorithm considering rigorous criteria for primers selection. Unlike alternative programs, LAMPrimers iQ can process long DNA or RNA sequences, and completely avoid primers that can form homo- …
A colorimetric RT-LAMP assay and LAMP-sequencing for
During the early phase of the COVID-19 pandemic (early March 2020) in Germany, we tested the sensitivity and specificity of a colorimetric RT-LAMP assay for detecting SARS-CoV-2 RNA in clinical RNA samples isolated from pharyngeal swab specimens collected from individuals being tested for COVID-19 (and provided by the Heidelberg University ...
Improving RT-LAMP detection of SARS-CoV-2 RNA through primer …
2022年4月2日 · Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has emerged as a viable molecular diagnostic method to expand the breadth and reach of nucleic acid testing, particularly for SARS-CoV-2 detection and surveillance. While rapidly growing in prominence, RT-LAMP remains a relativel …
NEB LAMP
NEB LAMP Primer Design tool can be used to design primers for LAMP reactions.