Oligo(dT) and Random DNA Primers - Thermo Fisher Scientific
Learn about the different pre-synthesized DNA primers and oligos available, including random primers and hexamers, oligo (dT) primers, and sequencing primers.
Oligo(dT) 20 Primer - Thermo Fisher Scientific
Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of 50C, Oligo(dT)20 Primer is a string of 20 deoxythymidylic acid residues that hybridizes to the poly(A) tail of mRNA.
Reverse Transcription Reaction Setup - Thermo Fisher Scientific
Oligo(dT) primers may be modified to improve efficiency of reverse transcription. For instance, the length of oligo(dT) primers may be extended to 20 nucleotides or longer to enable annealing in reverse transcription reactions at higher temperatures.
Oligo d(T)12-18 is the classic primer mix used to prime synthesis of the first strand cDNA by reverse transcriptase using poly A+ mRNA as a template. Oligo dT of various sizes are synthesized individually and gel purified.
Oligo dT vs. Random Primers - What's the Difference? | This vs. That
Oligo dT primers are short DNA sequences consisting of a string of thymine bases (T) followed by a few random nucleotides. They specifically bind to the poly-A tail of mRNA molecules, allowing for the synthesis of complementary DNA (cDNA) during reverse transcription.
Oligo dT Primers - Biocompare
Oligo dT primers are oligonucleotides that contain a segment of repeating deoxythymidines (dT). The dT anneal to the polyadenosine (polyA) tails of messenger RNA (mRNA), guiding the synthesis of complementary DNA (cDNA). Oligo dT primers are well-established reagents in cDNA synthesis and RT-PCR.
Random Primers Or Oligo(dT) Primers For cDNA Synthesis?
Oligo (dT) primers are designed so that they bind to the complementary poly (A) tails of messenger RNA (mRNA). Therefore, oligo (dT)s are only useful in cDNA reactions when mRNAs are the target for the downstream application. They will not anneal to non-polyA mRNA fragments, such as 18S rRNA.
Oligo (dT)20 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase, such as MMuLV or AMV. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)20 …
Oligo(dT)23, Anchored 70 uM in H 2 O Sigma-Aldrich
The Anchored Oligo(dT) 23 Primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures the oligo(dT) 23 primer binds at the beginning of the message such that there are no long regions of unusable sequence. Anchored oligo(dT) 23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA …
Oligo(dT) 15 Primer - Promega
Oligo(dT) 15 Primer is suitable for use as a primer for first-strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly(A) tail of mRNA.