Principle of the Test ASO latex agglutination is the rapid and simple test for the qualitative and semi-quantitative measurement of antibodies to Antistreptolysin-O in human serum. This method is based on an immunological reaction between streptococcal exoenzymes bound to biologically inert latex particles and streptococcal antibodies in the ...

Streptolysin O is an enzyme that lyses RBC membranes and is responsible for the zone of beta-hemolysis around colonies of group A streptococci seen on agar plates. Classically, ASO antibodies were detected by demonstrating serum’s capacity to inhibit or neutralize the lysis of RBC membranes by streptolysin O.

Principle for Antistreptolysin O titer (ASO) Lancefield group A streptococci are also known as streptococcus pyogenes. Group A β-streptococci produce various infections; the most common is acute pharyngitis.

Antistreptolysin O (ASO) titer is a blood test to measure antibodies against streptolysin O, a substance produced by group A streptococcus bacteria. Antibodies are proteins our bodies produce when they detect harmful substances, such as bacteria.