Short hairpin RNA - Wikipedia
A short hairpin RNA or small hairpin RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi).
Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of …
While siRNA delivers the siRNA duplex directly to the cytosol, shRNAs are capable of DNA integration and consist of two complementary 19–22 bp RNA sequences linked by a short loop of 4–11 nt similar to the hairpin found in naturally occurring miRNA.
What Is shRNA (Short-hairpin RNA)? - Genetic Education
2019年9月6日 · Often said as small hairpin RNA, the shRNA is a 20 to 25 bp polynucleotide chain of the RNA in which 4 to 11 nucleotides form a loop, a hairpin-like loop that binds to the mRNA molecule. Notedly, it lacks the dinucleotide overhang at the 3′ OH end, unlike the siRNA.
Short Hairpin RNA - an overview | ScienceDirect Topics
Short hairpin RNA (shRNA) is an alternative way to prepare siRNA sequences for delivery to cells that can be expressed in situ from plasmid DNA (pDNA) or from virus-derived constructs. The effectiveness of shRNA was first reported by Paddison and Hannon in 2002 [48].
Short Hairpin RNA - an overview | ScienceDirect Topics
Short/small hairpin RNAs (shRNAs) are single-stranded RNAs, which contain about 19-22 nucleotide base pairs; and linked with a short loop of 4-11 nucleotide base pairs that look similar to the hairpin present in miRNAs.
Short hairpin RNA (shRNA): design, delivery, and assessment of …
We provide suggestions for designing shRNA targets and controls, a protocol for sequencing through the secondary structure of the shRNA hairpin structure, and protocols for packaging and delivery of shRNA lentiviral particles.
Short hairpin RNAs (shRNAs) induce sequence-specific silencing …
2002年4月15日 · These small temporal RNAs (stRNAs) are transcribed as short hairpin precursors (approximately 70 nt), processed into active, 21-nt RNAs by Dicer, and recognize target mRNAs via base-pairing interactions.
Expressing short hairpin RNAs in vivo | Nature Methods
2006年8月23日 · Promoter-based expression of short hairpin RNAs (shRNAs) may in principle provide stable silencing of genes in any tissue. As for all approaches that require transgene expression, safe...
Short Hairpin RNA-Mediated Gene Silencing | SpringerLink
2012年1月1日 · These molecules, known as short hairpin RNAs (shRNAs), spontaneously form hairpin structures that are recognized by the cellular RNAi machinery and are processed to form active siRNAs.
shRNA - Applications - What is shRNA, how it works and its …
Short hairpin RNA (shRNA) sequences are usually encoded in a DNA vector that can be introduced into cells via plasmid transfection or viral transduction. shRNA molecules can be divided into two main categories based on their designs: …
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