Isolation and culture of neural stem cells from adult mouse ...
2022年3月18日 · Neural stem cells (NSCs) from the subventricular zone (SVZ) of the mouse brain can be expanded in vitro and grown as neurospheres, which can be stored long-term in liquid …
FACS isolation of endothelial cells and pericytes from mouse brain …
2018年3月22日 · Overview of V-SVZ dissection. Steps refer to numbers in Box 2. To dissect the V-SVZ from the brain, make 4 coronal cuts through the brain and discard the most rostral and …
Isolation of multipotent neural stem/progenitor cells from both …
Here we describe a protocol that enables us to isolate self-renewing and multipotent NSCs from the SVZ and the DG of the same adult mouse. The protocol involves the microdissection of the …
用于分离神经干细胞的鼠脑提取和脑室下区 （SVZ） 解剖 (视频)
2024年6月14日 · Murine Brain Extraction and Subventricular Zone (SVZ) Dissection for Isolation of Neural Stem Cells. J. Vis. Exp. (Pending Publication), e201941, doi:10.3791/201941 (2024).
Isolation and cultivation of neural stem cells | adult mouse brain ...
This application protocol describes a step-by-step protocol to isolate pure neural stem cells (NSCs) from the subventricular zone (SVZ) of adult mouse brain. Brain tissue from adult mice …
Isolation of multipotent neural stem or progenitor cells from both …
2012年10月18日 · The protocol involves the microdissection of the SVZ and DG from one adult mouse brain, isolation of NSCs from specific regions and cultivation of NSCs in vitro. The …
High-resolution mouse subventricular zone stem-cell niche …
2020年12月8日 · In the mouse brain, two distinct pools of NSC, found in the subventricular zone (SVZ) of the lateral ventricle walls and in the dentate gyrus of the hippocampus, produce new …
Isolation, Expansion, and Nucleofection of Neural Stem Cells from …
2024年6月14日 · Isolation and expansion of neural stem cells (NSCs) from the subventricular zone (SVZ) of the adult mouse brain can be achieved in a medium supplemented with basic …
The Subventricular Zone En-face: Wholemount Staining and …
2010年5月6日 · The walls of the lateral ventricles contain the largest germinal region in the adult mammalian brain. The subventricular zone (SVZ) in these walls is an extensively studied …
Genetic dissection of the glutamatergic neuron system in cerebral ...
2021年10月6日 · The listed genes are expressed in progenitor and PyN subpopulations. SVZ, subventricular zone; VZ, ventricular zone.