2019年3月1日 · In this study, we use correlative super resolution and electron microscopy to precisely determine where distal appendage proteins localize in relation to the centriole microtubules and...

Transmission of electron microscopy (TEM) of a cross-section of a centriole. Centrioles play a key role in organizing microtubules in the cytoplasm. During cell division, they are opposite poles of the spindle that separates chromosomes into daughter cells. They also form the basal bodies that give rise to cilia and flagella.

Figure 18 A centriole (at very high magnification): This micrograph represents a cross section of a centriole. The microtubules are shown in their characteristic arrangement.

Centrioles are vital cellular structures that organise centrosomes and cilia. Due to their subresolutional size, centriole ultrastructural features have been traditionally analysed by electron microscopy. Here we present an adaptation of magnified ...

Here, the authors use correlative 3D-superresolution, electron microscopy and electron tomography to detail the architecture of distal appendages, to describe the initial stages of appendage assembly and show that appendages of mature centrioles remodel during mitosis.

(A) Electron micrograph of a longitudinal section of centrioles in isolated centrosomes and cross sections highlighting the distal and subdistal appendages [adapted from Winey and O'Toole...

(C) Electron micrograph of a mature mother centriole with distal appendages (DA), subdistal appendages (SDA), and a cilium from a serum-starved RPE-1 cell. Ciliary axoneme (CA) is extending...

We identified only seven Journal of Cell Science 122 (20) cells that met the requirements to be identified as CPCs, and only three of them contained cross-sections suitable for understanding...

2021年2月1日 · In this mini review, we summarize the current knowledge of centriole architecture and propose a near-comprehensive model of the centriole architecture, compiling recent studies by cryo-electron tomography and regular electron microscopy on Chlamydomonas, Paramecium, Naegleria, Trichonympha, Teranympha and mammals [8, 11, 12, 14, 15]. We further ...

2024年12月24日 · Here, we use cryo-electron tomography to visualize centrosomes across the cell cycle in cells isolated from C. elegans embryos. We describe a pseudo-timeline of centriole assembly and identify distinct structural features in both mother and daughter centrioles.