2025年1月10日 · In single cells, variably sized nanoscale chromatin structures are observed, but it is unknown whether these form a cohesive framework that regulates RNA transcription. Here, we demonstrate that the human genome is an emergent, self …

Chromosome conformation capture-on-chip (4C) (also known as circular chromosome conformation capture) captures interactions between one locus and all other genomic loci. It involves a second ligation step, to create self-circularized DNA fragments, which are used to perform inverse PCR .

2018年4月23日 · Our work shows that chromatin conformation regulates the dynamic interplay between replication and transcription, unveiling its fundamental role in minimizing conflicts between the machineries...

Here, we discuss new findings, which have linked chromatin conformation with development, differentiation and diseases and hypothesized on various models while integrating all recent findings on how chromatin architecture affects gene …

In this review, we briefly introduce the molecular approaches, focusing on chromosome conformation capture or “3C” technology and its derivatives, which can be used to probe chromatin folding at resolutions beyond that provided by microscopy techniques.

2002年2月15日 · We developed a high-throughput methodology, Chromosome Conformation Capture (3C), which can be used to analyze the overall spatial organization of chromosomes and to investigate their physical properties at high resolution.

SEE is employed to investigate chromatin dynamics across different scales, enabling the detection of (i) rearrangements in topologically associating domains (TADs), and (ii) oscillations in chromatin interactions at gene loci.

5 天之前 · Since the emergence of high-throughput chromatin conformation capture (Hi-C) sequencing, researchers have worked to understand the mechanisms that result in the formation of topologically associated domains (TADs) and how these domains correspond to physical structures within individual cells (11–17). From their initial description as foci of ...

2018年4月23日 · Our work shows that chromatin conformation regulates the dynamic interplay between replication and transcription, unveiling its fundamental role in minimizing conflicts between the machineries moving along the same DNA template, and …

2021年9月3日 · Chromosome conformation capture (3C) assays are used to map chromatin interactions genome-wide. Chromatin interaction maps provide insights into the spatial organization of chromosomes...