Does Hoechst 33342 stain live cells?
Does Hoechst 33342 stain live cells?
Hoechst are non-intercalating dyes that bind the DNA minor groove at A–T-rich regions (Figure 1b). Hoechst dyes stain the DNA of both living and fixed cells and are compatible with…
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SOLVED: 83. Which of the following is false concerning the Acid Fast Stain? Mycoplasma species have a high content of waxy lipids in their cell wall, thus AFBs. B The waxy lipids in the wall of AFBs make them difficult to stain. C. Heat is used to drive carbol fuchsin (red dye) into cells - everything stained red. D. After washing, acid alcohol is used to destain everything but AFBs, followed by counterstaining with methylene blue (everything blue except red AFBs). E. All of the above are true o
SOLVED: 83. Which of the following is false concerning the Acid Fast Stain? Mycoplasma species have a high content of waxy lipids in their cell wall, thus AFBs. B The waxy lipids in the wall of AFBs make them difficult to stain. C. Heat is used to drive carbol fuchsin (red dye) into cells - everything stained red. D. After washing, acid alcohol is used to destain everything but AFBs, followed by counterstaining with methylene blue (everything blue except red AFBs). E. All of the above are true o
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