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The Folin Lowry method is one of the important methods of protein assay for determining the total amount of protein present in a given sample.
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SOLVED: Title: Determination of Protein Concentration by Lowry Method using Spectrophotometry TechniqueThe procedure being used in the experiment is the Lowry Method, which produces a blue-colored complex that absorbs maximum at 750nm. The intensity of the color produced is proportional to the protein concentration, allowing for quantitative estimation.The mechanism of the Lowry Method involves two parts. In the first part, the Biuret reagent is used. This reagent is made of copper(II) sulfate a
SOLVED: Title: Determination of Protein Concentration by Lowry Method using Spectrophotometry TechniqueThe procedure being used in the experiment is the Lowry Method, which produces a blue-colored complex that absorbs maximum at 750nm. The intensity of the color produced is proportional to the protein concentration, allowing for quantitative estimation.The mechanism of the Lowry Method involves two parts. In the first part, the Biuret reagent is used. This reagent is made of copper(II) sulfate a
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